Authors: I.A. Masiutin, E.Iu. Van, M.A. Smaga, J.S. Basova, V.G. Mikhailov

Title of the article: Study of the impact of pretreatment method of microcrystalline cellulose and its enzyme hydrolysis conditions on yield of reducing substances

Year: 2022, Issue: 1, Pages: 44-55

Branch of knowledge: 2.6.10. Organic matter technology

Index UDK: 663.534

DOI: 10.26730/1999-4125-2022-1-44-55

Abstract: Nowadays, an active search for alternative natural, ecological and environmentally friendly energy sources is being studied in connection with a complex of factors that complicate the recovery and processing of conventional fossil resources, and their negative impact on the environment. Lignocellulosic plant material is the most abundant polymer on Earth, and its reserves are practically inexhaustible. Cellulose obtained from lignocellulose is used as a model substrate for testing its conversion in fuel products. The study is aimed at the investigation of the impact of an enzyme composition and methods microcrystalline cellulose pretreatment on reducing substances yields. The research methods in the study were weight analysis to assess the yield of cellulose and lignin content; photometric method for the determination of α-cellulose; SEM to evaluate the results of chemical pretreatment of cellulose; photometric method for determining reducing substances. As a result, the effect of pretreatment of microcrystalline cellulose with various reagents (ZnCl2, NaOH, H3PO4) on the yield of reducing substances was studied. The optimal conditions for enzymatic hydrolysis for the composition "Cellulase" were found. The optimal ratio of the enzyme preparations "Cellulase" and "Xylanase" was experimentally established in order to maximize the yield of reducing substances, and all the studied samples were hydrolyzed with this mixture of enzymes in the established ratio. The content of reducing substances in terms of glucose in the hydrolyzates was determined by the photometric method by reaction with a copper-alkaline reagent. The principal technological scheme for the processing of cellulose-containing raw materials into products with high added value for fuel purposes is also proposed.

Key words: microcrystalline cellulose glucose reducing substances enzyme hydrolysis enzyme composition bioethanol chemical engineering of fuels biotechnology

Receiving date: 30.12.2021

Approval date: 30.01.2022

Publication date: 25.02.2022

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