Authors: I.A. Masiutin, E.Iu. Van, M.A. Smaga, J.S. Basova, V.G. Mikhailov

Title of the article: Study of miscanthus delignification methods for reducing substances production and economic aspects of its fuel-aimed processing

Year: 2022, Issue: 2, Pages: 19-30

Branch of knowledge: 2.6.10. Organic matter technology

Index UDK: 676.022, 662.7, 663.534

DOI: 10.26730/1999-4125-2022-2-19-30

Abstract: Plant raw materials are, nowadays, one of the most popular alternative energy sources. Among the wide variety of types of this raw material, miscanthus stands out for a number of competitive advantages, such as high yield per hectare and unpretentiousness during cultivation. In this regard, the purpose of this work was a comparative study of a number of methods for its delignification in order to obtain a cellulose-containing product and its further hydrolysis with a mixture of enzyme preparations, as well as a feasibility study of this multi-stage process. The research methods in the work were weight analysis to assess the yield of cellulose and lignin content; photometric method for the determination of α-cellulose; SEM to assess miscanthus delignification results; photometric method for reducing substances quantification. As a result, the influence of methods of alkaline, acid integrate delignification of Miscanthus sinensis Zebrinus on the yield of reducing substances from cellulose samples obtained as a result of this process was studied. The objects of comparison were samples of the original untreated miscanthus. Enzymatic hydrolysis was carried out with a mixture of preparations "Cellulase" and "Xylanase" under optimal conditions, experimentally selected by the authors in the previous study. As a result of the feasibility study, it was found that the most energy-consuming stages are the stage of mechanical grinding and enzymatic hydrolysis, the longest stage is the chemical delignification of miscanthus samples, including the stage of their preparation. Preliminary feasibility study of fuel bioethanol production shows the competitiveness of miscanthus processing technology with pretreatment with nitric acid and prehydrolysis with peracetic acid due to the reduction of the cost of commercial products while maintaining high rates of through extraction of the target product from the feedstock

Key words: miscanthus cellulose glucose reducing substances reducing sugars enzymatic hydrolysis enzyme composition bioethanol chemical engineering of fuels biotechnology

Receiving date: 17.02.2022

Approval date: 30.03.2022

Publication date: 29.04.2022

CITE DOWNLOAD